VISTA/B7-H5 (AA 33-194) protein (Fc Tag)

Catalog No. ABIN5564352

This Recombinant VISTA/B7-H5 protein is produced in HEK-293 Cells.

Quick Overview for VISTA/B7-H5 (AA 33-194) protein (Fc Tag) (ABIN5564352)

Target: VISTA/B7-H5

Protein Type: Recombinant

Origin: Human, Mouse

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 33-194

Purification tag / Conjugate: Fc Tag

Purpose: VISTA [B7-H5] (human):Fc (human) (rec.)

Cross-Reactivity: Human, Mouse

Characteristics: The extracellular domain of human VISTA (aa 33-194) is fused at the C-terminus to the Fc portion of human IgG1.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: VISTA/B7-H5

Alternative Name: VISTA [B7-H5]

Background:

V-type Immunoglobulin Domain-containing Suppressor of T Cell Activation, Platelet Receptor Gi24, Stress-induced Secreted Protein-1, Sisp-1, V-set Domain-containing Immunoregulatory Receptor

Human VISTA is a 55- to 65 kDa type I Ig membrane protein with the extracellular domain homologous to PD-L1. VISTA is mainly expressed on hematopoietic tissues (spleen, thymus and bone marrow) and on myeloid cells with lower expression on CD4+ and CD8+ T cells. VISTA is a new negative checkpoint regulator that potently suppresses T cell activation. Overexpression of VISTA on tumors inhibits the protective anti-tumor immunity and blockade of VISTA enhances antitumor immunity in multiple tumor models. VISTA on APC delivers negative signaling to T cells via a yet-to-identify binding partner on T cells.

Molecular Weight: ~65-70kDa (SDS-PAGE)

UniProt: Q9H7M9