Neuregulin 4 Protein (NRG4) (AA 1-53) (GST tag)

Catalog No. ABIN5564359

This Recombinant Neuregulin 4 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Neuregulin 4 Protein (NRG4) (AA 1-53) (GST tag) (ABIN5564359)

Target: Neuregulin 4 (NRG4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 1-53

Purification tag / Conjugate: This Neuregulin 4 protein is labelled with GST tag.

Purpose: Neuregulin-4 (mouse) (rec.)

Cross-Reactivity: Human, Mouse

Characteristics: Mouse Neuregulin-4 (aa 1-53) is fused at the N-terminus to a GST-tag.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Binds to mouse and human receptor tyrosine-protein kinase ErbB4.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: Neuregulin 4 (NRG4)

Alternative Name: Neuregulin-4

Background:

Nrg4

Neuregulin-4 (Nrg4) belongs to a small family of EGF-like (EGFL) domain-containing proteins that are synthesized as transmembrane precursors and undergo proteolytic cleavage. The EGF-like domain (aa 5-46) of Nrg4 (aa 1-53) directly binds to the receptors ErbB3 and 4. Nrg4 is a cold induced adipokine, highly expressed in adipose tissues and enriched in brown fat. It is increased during brown adipocyte differentiation and reduced in rodent and human obesity. It promotes neurite outgrowth and protects against diet-induced insulin resistance and hepatic steatosis through attenuating hepatic lipogenic signaling. This hepatic effect of Nrg4 is mediated by ErbB3 and ErbB4 signaling that negatively regulates de novo lipogenesis mediated by LXR and SREBP1c. This effect of Nrg4 on fatty liver and insulin resistance could lead to the development of Nrg4 as an effective therapeutic biological for the treatment of NAFLD and type 2 diabetes. GST-Nrg4 (aa 1-53) recombinant protein has been shown to mimic the effect of endogenous secreted Nrg4 on liver lipogenesis.

Molecular Weight: ~32kDa (SDS-PAGE)

UniProt: Q9WTX4

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway