Resistin Protein (RETN) (AA 19-108) (DYKDDDDK Tag)
Quick Overview for Resistin Protein (RETN) (AA 19-108) (DYKDDDDK Tag) (ABIN5564365)
Target
See all Resistin (RETN) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 19-108
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Purification tag / Conjugate
- This Resistin protein is labelled with DYKDDDDK Tag.
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Purpose
- Resistin (human) (rec.)
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Cross-Reactivity
- Human
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Characteristics
- Human resistin (aa 19-108) is fused at the N-terminus to a FLAG®-tag.
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Endotoxin Level
- <0.01EU/μg purified protein (LAL test).
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Biological Activity Comment
- Inhibits insulin-stimulated glucose uptake in L6 skeletal muscle cells.
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Resistin (RETN) (Active) protein
RETN Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Resistin (RETN) (AA 21-114) (Active) protein (hFc Tag)RETN Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Resistin (RETN) (AA 17-108) (Active) protein (hFc Tag)RETN Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Resistin (RETN) (AA 1-108) protein (GST tag)RETN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Resistin (RETN) (AA 19-108) protein (Fc Tag)RETN Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Resistin (RETN) (AA 16-108) protein (His-IF2DI Tag)RETN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Resistin (RETN) protein (Myc-DYKDDDDK Tag)RETN Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Concentration
- Lot specific
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Buffer
- Contains PBS.
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Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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Expiry Date
- 6 months
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- Resistin (RETN)
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Alternative Name
- Resistin
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Background
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FIZZ3, Found in Inflammatory Zone 3, ADSF, Adipose Tissue-Specific Secretory Factor
The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM, RELM-alpha/FIZZ 1 and RELM-beta/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM.
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Molecular Weight
- ~13kDa (SDS-PAGE)
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UniProt
- Q9HD89
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Pathways
- Feeding Behaviour, Smooth Muscle Cell Migration
Target
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