IDO1 Protein (AA 1-403) (His tag)

Catalog No. ABIN5564386

This Recombinant IDO1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IDO1 Protein (AA 1-403) (His tag) (ABIN5564386)

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 1-403

Purification tag / Conjugate: This IDO1 protein is labelled with His tag.

Purpose: IDO (human) (rec.) (His) (highly active)

Cross-Reactivity: Human

Characteristics: Human IDO1 (aa 1-403) is fused at the N-terminus to a His-tag.

Sterility: Sterile filtered

Endotoxin Level: <0.1EU/μg purified protein (LAL test).

Biological Activity Comment: Specific Activity: >100'000U/mg protein with L-tryptophan as substrate (activity assay with catalase). One unit is defined as the amount of enzyme that produces 1nmol of N-formylkynurenine (NFK) per hour. For more information see Activity Test Protocol.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.2μm-filtered solution in 10 mM Tris, pH 7.5, containing 100 mM NaCl and 20 % glycerol.

Handling Advice: After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 1 year after receipt when stored at -80°C.

Expiry Date: 12 months

Target Details

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Alternative Name: IDO

Background:

Indoleamine 2,3-dioxygenase 1, INDO, IDO

IDO1 is a heme enzyme that catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway, causing depletion of tryptophan which can lead to halted growth of microbes as well as T cells. IDO1 is an immune checkpoint protein, thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation and antioxidant activity. Cancer cells are able to evade the immune system is by hijacking the checkpoint proteins. Increased IDO1 protein levels drive growth arrest and apoptosis of the effector T cells, a group of immune cells that mediate the immune system's ability to destroy pathogens. By reducing the number of effector T cells, IDO1 overexpression prevents the immune system from effectively destroying cancer cells. IDO1 overexpression has been observed in a wide range of human cancers such as prostatic, colorectal, pancreatic, cervical, gastric, ovarian, head or lung cancer. Physiological IDO1 activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders. Biological active IDO1 enzyme can be used for screening of IDO1 inhibitors, which have shown promising antitumor activity and appear to be synergistic in combination with chemotherapy and other forms of immunotherapy.

Molecular Weight: ~47kDa (SDS-PAGE)

UniProt: P14902

Pathways: Activated T Cell Proliferation