PARP1 Protein (E988K) (HA tag,His tag)

Catalog No. ABIN5564467

This Recombinant PARP1 protein is expressed in Insect cells (Sf21).

Quick Overview for PARP1 Protein (E988K) (HA tag,His tag) (ABIN5564467)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Insect cells (Sf21)

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: E988K

Purification tag / Conjugate: This PARP1 protein is labelled with HA tag,His tag.

Purpose: PARP-1 [ARTD1] (E998K Mutant) (human) (rec.) Control

Cross-Reactivity: Human

Characteristics: Human full-length inactive mutant E988K of PARP-1 is fused to a HA-tag and a His-tag.

Biological Activity Comment: 0.5% of wild type PARP-1.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride and 50 mM imidazole, 0.2 % NP-40 and 10 % glycerol.

Handling Advice: After opening, prepare aliquots and store at -80 °C. Avoid freeze/thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.

Expiry Date: 6 months

Target Details

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP-1 [ARTD1]

Background:

Poly(ADP-ribose) Polymerase 1, ADP-ribosyltransferase Diphtheria Toxin-like 1, NAD(+) ADP-ribosyltransferase 1

PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E988K mutant) is an inactive form of PARP-1 which can be used as a control compound.

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location