AZGP1 Protein (AA 21-298)

Catalog No. ABIN5564470

This Recombinant AZGP1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for AZGP1 Protein (AA 21-298) (ABIN5564470)

Target: AZGP1 (alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 21-298

Purpose: Zinc-alpha-2-glycoprotein (human) (rec.) (untagged)

Cross-Reactivity: Human

Characteristics: Human full-length zinc-alpha-2-glycoprotein (aa 21-298) is untagged.

Endotoxin Level: <0.1EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: AZGP1 (alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1))

Alternative Name: Zinc-alpha-2-glycoprotein

Background:

Zn-alpha-2-GP, Zn-alpha-2-Glycoprotein, ZAG, ZA2G, AZGP1, ZNGP1

Zinc-alpha-2-glycoprotein (ZAG), first identified in the 1960s, derives its name from its precipitation from human plasma upon the addition of zinc salts. ZAG has since been found in secretory epithelial cells and in a range of body fluids. ZAG is identical to a lipid mobilizing factor isolated from the urine of patients with cancer cachexia and stimulates lipolysis in in vitro and in vivo experiments. Due to its expression in, and secretion from adipocytes, ZAG is considered an adipokine. Recently the clinical significance of ZAG has been clarified. ZAG expression in adipocytes is inversely related to fat mass, thus it is intimately involved in the maintenance of body weight in mice and humans. Epidemiological studies have uncovered an association between ZAG and plasma cholesterol. The non-synonymous single nucleotide polymorphism rs4215 in ZAG is associated with plasma cholesterol and obesity. Structurally ZAG possesses a class I major histocompatibility complex (MHC) protein fold. It is distinct from other members of this protein family in that it is soluble, rather than being anchored to plasma membranes, and it associates with prolactin inducible protein rather than beta2-microglobulin. Similar to peptide antigen-presenting class I MHC molecules, ZAG possesses an open apical groove between its alpha1 and alpha2 domain helices.

Molecular Weight: ~30kDa

UniProt: P25311

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process