TIGIT Protein (AA 22-138) (Fc Tag)

Catalog No. ABIN5564495

Recombinant TIGIT protein expressed in HEK-293 Cells.

Quick Overview for TIGIT Protein (AA 22-138) (Fc Tag) (ABIN5564495)

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 22-138

Purification tag / Conjugate: This TIGIT protein is labelled with Fc Tag.

Purpose: TIGIT (human):Fc (human) (rec.)

Cross-Reactivity: Human

Characteristics: The extracellular domain of human TIGIT (aa 22-138) is fused at the C-terminus to the Fc portion of human IgG1.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT

Background:

T Cell Immunoreceptor with Ig and ITIM Domains, V-set and Immunoglobulin Domain-containing Protein 9, V-set and Transmembrane Domain-containing Protein 3, Vstm3, VSIG9, WUCAM

TIGIT (T cell immunoreceptor with Ig and ITIM domains) is a novel member of the immunoglobulin super family (IgSF). TIGIT and CD226 bind the same ligands, the two nectin-family members poliovirus receptor (PVR) and poliovirus receptor-related 2 (CD112) and compete for each other. TIGIT inhibits while CD226 activates the signaling through PVR and CD112. TIGIT and CD226 directly interact at the cell surface and this interaction impairs CD226 homodimerization and function. TIGIT is absent on naive T cells, but is expressed on activated Tregs and memory T cells, and on NK cells and NKT cells. TIGIT negatively regulates T cell activation and blockade of TIGIT exacerbates several immune diseases. TIGIT also activates the PVR receptor on dendritic cells (DCs). Addition of TIGIT (human):Fc (human) to DCs triggers phosphorylation of PVR and elicits downstream signaling, which then inhibits T cell responses by producing IL-10. TIGIT also negatively regulates NK cell cytotoxicity and cytokine production. TIGIT is directly regulated by FoxP3 and promotes inducible Treg differentiation.

Molecular Weight: ~40-45kDa (SDS-PAGE)

UniProt: Q495A1

Pathways: Cancer Immune Checkpoints