CD14 Protein (CD14) (His tag)

Catalog No. ABIN5663349

Recombinant CD14 protein expressed in CHO Cells.

Quick Overview for CD14 Protein (CD14) (His tag) (ABIN5663349)

Target: CD14

Protein Type: Recombinant

Origin: Human

Source: CHO Cells

Application: Western Blotting (WB), ELISA, Agonist (Agon), Dot Blot (DB)

Purity: >95 % by SDS-PAGE

Product Details

Purification tag / Conjugate: This CD14 protein is labelled with His tag.

Sequence: MERASCLLLL LLPLVHVSAT TPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV PACARSTLSV G VSGTLVLLQG ARGFA

Characteristics: The hCD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR.

Purification: affinity chromatography with monoclonal CD14 antibody biG2

Endotoxin Level: <1EU/μg of recombinant human CD14 as determined by LAL method

Biological Activity Comment: Up to 20 μg/ml CD14 inhibit binding of FITC-LPS (0.5μg/ml) to 6 x 105 CD14+CHO transfectants (FACS)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 40 μL sterile distilled water to a concentration of 0.25 mg/mL

Concentration: 0.25 mg/mL

Buffer: sterile-filtered phosphate balanced salt solution, pH 7.2

Preservative: Without preservative

Storage: RT,-20 °C,-80 °C

Storage Comment:

Storage: -20°C or -70°C

Storage/Stability: Long time storage at -70oC. When stored at recommended temperature, this protein is stable for 12 months. The lyophilized product thought stable room temperature up to 3 weeks, but is best stored desiccated at -70oC more than 1 year, at refrigerator about 10 months. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20oC. For long-term storage it is recommended to add a carrier protein (o.1% BSA). Repeated freezing and thawing should be avoided !!!

Expiry Date: 12 months

Target Details

Target: CD14

Alternative Name: CD14

Background: Background: CD14 acts as a receptor for endotoxin (LPS), expressed strongly on monocytes, macrophages and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.The rhuCD14 was produced in human CD14 transfected CHO-cells in serum free medium and has been purified by affinity chromatography with monoclonal antibody to hCD14 biG2at column and controlled by SDS page (90-95 % purity). Before transfection, the complete human CD14-cDNA amplified by PCR and cloned into expression vector p-POL-DHFR. CD14 is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, also expressed by most tissue macrophages.

Molecular Weight: ~50kDa

Gene ID: 929

NCBI Accession: NP_000582

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades