JAK2 Protein (AA 752-1132, partial) (His tag)

Catalog No. ABIN5709779

This Recombinant JAK2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for JAK2 Protein (AA 752-1132, partial) (His tag) (ABIN5709779)

Target: JAK2 (Janus Kinase 2 (JAK2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 752-1132, partial

Purification tag / Conjugate: This JAK2 protein is labelled with His tag.

Sequence: KPLSALDSQR KLQFYEDRHQ LPAPKWAELA NLINNCMDYE PDFRPSFRAI IRDLNSLFTP DYELLTENDM LPNMRIGALG FSGAFEDRDP TQFEERHLKF LQQLGKGNFG SVEMCRYDPL QDNTGEVVAV KKLQHSTEEH LRDFEREIEI LKSLQHDNIV KYKGVCYSAG RRNLKLIMEY LPYGSLRDYL QKHKERIDHI KLLQYTSQIC KGMEYLGTKR YIHRDLATRN ILVENENRVK IGDFGLTKVL PQDKEYYKVK EPGESPIFWY APESLTESKF SVASDVWSFG VVLYELFTYI EKSKSPPAEF MRMIGNDKQG QMIVFHLIEL LKNNGRLPRP DGCPDEIYMI MTECWNNNVN QRPSFRDLAL RVDQIRDNMA G

Purification: SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: JAK2 (Janus Kinase 2 (JAK2))

Alternative Name: JAK2

Background: Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO), or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.

Molecular Weight: 48.7 kDa

UniProt: O60674

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response