Vpx Protein (VPX) (AA 1-112), (full length) protein (His-SUMO Tag)

Catalog No. ABIN5710778

This Recombinant Vpx Protein protein is expressed in Escherichia coli (E. coli).

Quick Overview for Vpx Protein (VPX) (AA 1-112), (full length) protein (His-SUMO Tag) (ABIN5710778)

Target: Vpx Protein (VPX)

Protein Type: Recombinant

Origin: Simian Immunodeficiency Virus (SIV)

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 1-112, full length

Purification tag / Conjugate: His-SUMO Tag

Sequence: MSDPRERIPP RNSGEETIGE AFEWLNRTVE EINREAVNHL PRELIFQVWQ RSWEYWHDEQ RMSQSYVKYR YLCLMQKALF MHCKKGCRCL GEGHRAGGWR PGPPPPPPPG LA

Purification: SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: Vpx Protein (VPX)

Alternative Name: VPX

Target Type: Viral Protein

Background: Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription .

Molecular Weight: 29.21 kDa

UniProt: P11266