BtuF (AA 24-266) protein (GST tag)

Catalog No. ABIN5712884

This Recombinant BtuF protein is produced in Escherichia coli (E. coli).

Quick Overview for BtuF (AA 24-266) protein (GST tag) (ABIN5712884)

Target: BtuF

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 24-266

Purification tag / Conjugate: GST tag

Purpose: Recombinant Escherichia coli Vitamin B12-binding protein(btuF)

Sequence: PRVITLSPAN TELAFAAGIT PVGVSSYSDY PPQAQKIEQV STWQGMNLER IVALKPDLVI AWRGGNAERQ VDQLASLGIK VMWVDATSIE QIANALRQLA PWSPQPDKAE QAAQSLLDQY AQLKAQYADK PKKRVFLQFG INPPFTSGKE SIQNQVLEVC GGENIFKDSR VPWPQVSREQ VLARSPQAIV ITGGPDQIPK IKQYWGEQLK IPVIPLTSDW FERASPRIIL AAQQLCNALS QVD

Characteristics: N-terminal GST-tagged

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: Tris-based buffer50 % glycerol

Storage: -20 °C,-80 °C

Storage Comment: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Expiry Date: 6-12 months

Target Details

Target: BtuF

Background: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC.

Molecular Weight: 53.9 kDa

Gene ID: 947574

UniProt: P37028