Estrogen Receptor alpha Protein (AA 9-591, partial) (His tag)

Catalog No. ABIN5713438

Product Details

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Protein Type: Recombinant

Protein Characteristics: partial, AA 9-591

Origin: Human

Source: Yeast

Purification tag / Conjugate: This Estrogen Receptor alpha protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: ASGMALLHQI QGNELEPLNR PQLKIPLERP LGEVYLDSSK PAVYNYPEGA AYEFNAAAAA NAQVYGQTGL PYGPGSEAAA FGSNGLGGFP PLNSVSPSPL MLLHPPPQLS PFLQPHGQQV PYYLENEPSG YTVREAGPPA FYRPNSDNRR QGGRERLAST NDKGSMAMES AKETRYCAVC NDYASGYHYG VWSCEGCKAF FKRSIQGHND YMCPATNQCT IDKNRRKSCQ ACRLRKCYEV GMMKGGIRKD RRGGRMLKHK RQRDDGEGRG EVGSAGDMRA ANLWPSPLMI KRSKKNSLAL SLTADQMVSA LLDAEPPILY SEYDPTRPFS EASMMGLLTN LADRELVHMI NWAKRVPGFV DLTLHDQVHL LECAWLEILM IGLVWRSMEH PGKLLFAPNL LLDRNQGKCV EGMVEIFDML LATSSRFRMM NLQGEEFVCL KSIILLNSGV YTFLSSTLKS LEEKDHIHRV LDKITDTLIH LMAKAGLTLQ QQHQRLAQLL LILSHIRHMS NKGMEHLYSM KCKNVVPLYD LLLEMLDAHR LHAPTSRGGA SVEETDQSHL ATAGSTSSHS LQKYYITGEA EGF

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ESR1 (ESR1 Products)

Synonyms: LOC398734 Protein, AA420328 Protein, AU041214 Protein, ER-alpha Protein, ER[a] Protein, ERa Protein, ERalpha Protein, ESR Protein, Estr Protein, Estra Protein, Nr3a1 Protein, NR3A1 Protein, eralpha Protein, zfER[a] Protein, ER Protein, ESRA Protein, ESTRR Protein, Era Protein, akap12 Protein, cb27 Protein, esr1 Protein, fb72g12 Protein, id:ibd1202 Protein, sb:cb27 Protein, si:ch73-192g21.1 Protein, wu:fb72g12 Protein, wu:fc21c03 Protein, Esr Protein, RNESTROR Protein, ERALPHA Protein, er Protein, esr Protein, nr3a1 Protein, XER Protein, era Protein, esra Protein, ERbeta Protein, xesr-1 Protein, xlERalpha1 Protein, xlERalpha2 Protein, ESR1 Protein, estrogen receptor 1 L homeolog Protein, estrogen receptor 1 (alpha) Protein, estrogen receptor 1 Protein, A kinase (PRKA) anchor protein 12b Protein, esr1.L Protein, Esr1 Protein, ESR1 Protein, esr1 Protein, akap12b Protein

Background: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response elent (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response elent of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elents, the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates mbrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1

Molecular Weight: 66.9 kDa

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly