Afadin Protein (MLLT4) (AA 1-141, Ras Binding Domain)

Catalog No. ABIN5777013

This Recombinant Afadin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Afadin Protein (MLLT4) (AA 1-141, Ras Binding Domain) (ABIN5777013)

Target: Afadin (MLLT4)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 1-141, Ras Binding Domain

Purpose: AF6-RBDGST (residues 1-141), GTP-H-Ras binding cytoplasmic protein, Afadin - Ras-binding domain, human, recombinant, E. coli

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied in 25 mM HEPES pH 7.550 mM NaCl1 mM EDTA and 50 % glycerol

Preservative: Without preservative

Handling Advice: Please centrifuge briefly before opening (volume ≤2 ml).,avoid freeze/thaw cycles

Storage: -20 °C

Storage Comment: store at -20 °C avoid freeze/thaw cycles

Expiry Date: 12 months

Target Details

Target: Afadin (MLLT4)

Alternative Name: AF6

Background: The amino terminus of Af6 was identified as the Rasbinding site. Additionally to the Ras-binding Af6 also binds Rap. ZO-1, a protein involved in the formation of tight junctions, also binds to Af6 close to the amino terminus thereby competing with Ras binding. These data suggest a participation of Af6 in the regulation of cell-cell contacts via a Ras-modulated interaction with ZO-1. In contrast to the well characterized effectors Raf, RalGEF, and PI(3)kinase, Af6 is a protein with no enzymatic function. It seems to serve as a scaffolding component for protein complexes. This would represent a new type of Ras effector because Ras binding in this case does not lead to the activation of the enzymatic activity of the effector.

NCBI Accession: NM_005936

Pathways: Cell-Cell Junction Organization