Interferon gamma Protein (IFNG) (AA 24-161)
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- Target See all Interferon gamma (IFNG) Proteins
- Interferon gamma (IFNG)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 24-161
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS), Activity Assay (AcA)
- Sequence
- MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG
- Purity
- > 90% by SDS-PAGE
- Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Measured in a cytotoxicity assay using WiDr cells. The ED50 range ≤ 0.5ng/ml.
- Top Product
- Discover our top product IFNG Protein
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Interferon gamma (IFNG) (AA 24-161) protein (His-Avi Tag)
IFNG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interferon gamma (IFNG) (AA 24-161) protein (His-Avi Tag,Biotin)IFNG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interferon gamma (IFNG) (Active) proteinIFNG Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interferon gamma (IFNG) (AA 23-155) (Active) proteinIFNG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as analyzed by reducing SDS-PAGE. Active
Interferon gamma (IFNG) proteinIFNG Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Interferon gamma (IFNG) (AA 24-166) protein (His tag)IFNG Origin: Pig Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interferon gamma (IFNG) (AA 1-144) protein (His tag)IFNG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interferon gamma (IFNG) (AA 24-174) protein (His tag)IFNG Origin: Hamster Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interferon gamma (IFNG) (AA 24-167) protein (His tag)IFNG Origin: Cat Host: Escherichia coli (E. coli) Recombinant > 95% by SDS-PAGE SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In PBS buffer ( pH 7.4) containing 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- Interferon gamma (IFNG)
- Alternative Name
- IFN-gamma/IFNG (IFNG Products)
- Background
- IFNG is a member of the type II interferon family. This protein is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia. Recombinant human IFNG protein was expressed in E. coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 16.3 kDa (139aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_000610
- UniProt
- P01579
- Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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