Interferon gamma Protein (IFNG) (AA 24-161)
Quick Overview for Interferon gamma Protein (IFNG) (AA 24-161) (ABIN7529268)
Target
See all Interferon gamma (IFNG) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 24-161
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Sequence
- MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG
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Purification
- > 90% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured in a cytotoxicity assay using WiDr cells. The ED50 range ≤ 0.5ng/ml.
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Interferon gamma (IFNG) (AA 24-161) (Active) protein (Biotin,His-Avi Tag)
IFNG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interferon gamma (IFNG) (AA 24-161) (Active) protein (His-Avi Tag)IFNG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interferon gamma (IFNG) (AA 23-155) (Active) proteinIFNG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as analyzed by reducing SDS-PAGE. Active
Interferon gamma (IFNG) proteinIFNG Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func
Interferon gamma (IFNG) (AA 23-155) protein (His tag)IFNG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Interferon gamma (IFNG) (AA 24-161) (Active) protein (His tag)IFNG Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS, AcA Active
Interferon gamma (IFNG) (AA 24-174) protein (His tag)IFNG Origin: Hamster Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interferon gamma (IFNG) (AA 24-166) protein (His tag)IFNG Origin: Pig Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Interferon gamma (IFNG) (AA 24-166) (Active) proteinIFNG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE. WB, ELISA Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol,
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Interferon gamma (IFNG)
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Alternative Name
- IFN-gamma/IFNG
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Background
- IFNG is a member of the type II interferon family. This protein is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia. Recombinant human IFNG protein was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 16.3 kDa (139aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_000610
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Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
Target
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