CSK Protein (AA 1-450) (His tag)
Quick Overview for CSK Protein (AA 1-450) (His tag) (ABIN5853916)
Target
See all CSK ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-450
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Purification tag / Conjugate
- This CSK protein is labelled with His tag.
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Sequence
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MSAIQAA WPSGTECIAK YNFHGTAEQD LPFCKGDVLT IVAVTKDPNW YKAKNKVGRE GIIPANYVQK REGVKAGTKL SLMPWFHGKI TREQAERLLY PPETGLFLVR ESTNYPGDYT LCVSCDGKVE HYRIMYHASK LSIDEEVYFE NLMQLVEHYT SDADGLCTRL IKPKVMEGTV AAQDEFYRSG WALNMKELKL LQTIGKGEFG DVMLGDYRGN KVAVKCIKND ATAQAFLAEA SVMTQLRHSN LVQLLGVIVE EKGGLYIVTE YMAKGSLVDY LRSRGRSVLG GDCLLKFSLD VCEAMEYLEG NNFVHRDLAA RNVLVSEDNV AKVSDFGLTK EASSTQDTGK LPVKWTAPEA LREKKFSTKS DVWSFGILLW EIYSFGRVPY PRIPLKDVVP RVEKGYKMDA PDGCPPAVYE VMKNCWHLDA AMRPSFLQLR EQLEHIKTHE LHL -
Purification
- > 95% by SDS-PAGE
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C-Src tyrosine Kinase (CSK) (AA 1-450) protein (GST tag)
CSK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
C-Src tyrosine Kinase (CSK) (Active) protein (GST tag,His tag)CSK Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE Active
C-Src tyrosine Kinase (CSK) (Active) protein (GST tag)CSK Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 92 % as determined by reducing SDS-PAGE. Active
C-Src tyrosine Kinase (CSK) proteinCSK Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
C-Src tyrosine Kinase (CSK) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)CSK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 20 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- CSK (C-Src tyrosine Kinase (CSK))
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Alternative Name
- CSK
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Background
- CSK is a tyrosine-protein kinase CSK that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.. Recombinant human CSK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 53.1 kDa (473aa)
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NCBI Accession
- NP_001120662
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Pathways
- TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events
Target
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