CSK Protein (AA 1-450) (GST tag)
(1 reference)-
- Target See all CSK Proteins
- CSK (C-Src tyrosine Kinase (CSK))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-450
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This CSK protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- CSK (Human) Recombinant Protein (P01)
- Sequence
- MSAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA KNKVGREGII PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLYPPE TGLFLVREST NYPGDYTLCV SCDGKVEHYR IMYHASKLSI DEEVYFENLM QLVEHYTSDA DGLCTRLIKP KVMEGTVAAQ DEFYRSGWAL NMKELKLLQT IGKGEFGDVM LGDYRGNKVA VKCIKNDATA QAFLAEASVM TQLRHSNLVQ LLGVIVEEKG GLYIVTEYMA KGSLVDYLRS RGRSVLGGDC LLKFSLDVCE AMEYLEGNNF VHRDLAARNV LVSEDNVAKV SDFGLTKEAS STQDTGKLPV KWTAPEALRE KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDAPDG CPPAVYEVMK NCWHLDAAMR PSFLQLREQL EHIKTHELHL
- Characteristics
- Human CSK full-length ORF ( NP_004374.1, 1 a.a. - 450 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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C-Src tyrosine Kinase (CSK) (AA 1-100) protein (GST tag)
CSK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
C-Src tyrosine Kinase (CSK) (AA 1-450) protein (His tag)CSK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
C-Src tyrosine Kinase (CSK) proteinCSK Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
C-Src tyrosine Kinase (CSK) (Active) protein (GST tag)CSK Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 92 % as determined by reducing SDS-PAGE. Active
C-Src tyrosine Kinase (CSK) (Active) protein (GST tag,His tag)CSK Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE Active
C-Src tyrosine Kinase (CSK) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)CSK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "SHP-2 binds to caveolin-1 and regulates Src activity via competitive inhibition of CSK in response to H2O2 in astrocytes." in: PLoS ONE, Vol. 9, Issue 3, pp. e91582, (2014) (PubMed).
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: "SHP-2 binds to caveolin-1 and regulates Src activity via competitive inhibition of CSK in response to H2O2 in astrocytes." in: PLoS ONE, Vol. 9, Issue 3, pp. e91582, (2014) (PubMed).
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- Target
- CSK (C-Src tyrosine Kinase (CSK))
- Alternative Name
- CSK (CSK Products)
- Synonyms
- AW212630 Protein, C-terminal Src kinase Protein, c-src tyrosine kinase Protein, CSK, non-receptor tyrosine kinase Protein, CSK Protein, Csk Protein
- Background
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Full Gene Name: c-src tyrosine kinase
Synonyms: MGC117393 - Gene ID
- 1445
- NCBI Accession
- NM_004383
- Pathways
- TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events
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