EPH Receptor A2 Protein (EPHA2) (AA 27-537) (His tag)
Quick Overview for EPH Receptor A2 Protein (EPHA2) (AA 27-537) (His tag) (ABIN7281133)
Target
See all EPH Receptor A2 (EPHA2) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 27-537
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Purification tag / Conjugate
- This EPH Receptor A2 protein is labelled with His tag.
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Sequence
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KEVVLLD FAAAGGELGW LTHPYGKGWD LMQNIMNDMP IYMYSVCNVM SGDQDNWLRT NWVYRGEAER IFIELKFTVR DCNSFPGGAS SCKETFNLYY AESDLDYGTN FQKRLFTKID TIAPDEITVS SDFEARHVKL NVEERSVGPL TRKGFYLAFQ DIGACVALLS VRVYYKKCPE LLQGLAHFPE TIAGSDAPSL ATVAGTCVDH AVVPPGGEEP RMHCAVDGEW LVPIGQCLCQ AGYEKVEDAC QACSPGFFKF EASESPCLEC PEHTLPSPEG ATSCECEEGF FRAPQDPASM PCTRPPSAPH YLTAVGMGAK VELRWTPPQD SGGREDIVYS VTCEQCWPES GECGPCEASV RYSEPPHGLT RTSVTVSDLE PHMNYTFTVE ARNGVSGLVT SRSFRTASVS INQTEPPKVR LEGRSTTSLS VSWSIPPPQQ SRVWKYEVTY RKKGDSNSYN VRRTEGFSVT LDDLAPDTTY LVQVQALTQE GQGAGSKVHE FQTLSPEGSG NLAV -
Purification
- > 95% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA with Human Ephrin-A1 (CAT# ATGP3782).
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EPH Receptor A2 (EPHA2) (AA 24-537) (Active) protein (His tag)
EPHA2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
EPH Receptor A2 (EPHA2) (AA 26-538) (Active) protein (His tag)EPHA2 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
EPH Receptor A2 (EPHA2) (AA 24-534) (Active) protein (His-Avi Tag)EPHA2 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
EPH Receptor A2 (EPHA2) (AA 24-534) (Active) protein (hFc Tag)EPHA2 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
EPH Receptor A2 (EPHA2) (AA 24-534) protein (His tag)EPHA2 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
EPH Receptor A2 (EPHA2) (AA 26-535) protein (His tag)EPHA2 Origin: Mouse Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
EPH Receptor A2 (EPHA2) (AA 204-326) protein (GST tag)EPHA2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
EPH Receptor A2 (EPHA2) (AA 1-976) protein (GST tag)EPHA2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
EPH Receptor A2 (EPHA2) (AA 585-976) (Active) protein (GST tag,His tag)EPHA2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
EPH Receptor A2 (EPHA2) (Active) protein (His tag)EPHA2 Origin: Mouse Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- EPH Receptor A2 (EPHA2)
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Alternative Name
- EphA2
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Background
- EPHA2, also known as ephrin type-A receptor 2, is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. It is implicated as positional labels that may guide the development of neural topographic maps. It has also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization. Recombinant human EPHA2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques
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Molecular Weight
- 57.3 kDa (520aa)
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NCBI Accession
- NP_004422
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Pathways
- RTK Signaling
Target
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