BCAM Protein (AA 32-547) (hIgG-His-tag)
Quick Overview for BCAM Protein (AA 32-547) (hIgG-His-tag) (ABIN5854904)
Target
See all BCAM ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 32-547
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Purification tag / Conjugate
- This BCAM protein is labelled with hIgG-His-tag.
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Sequence
- EVRLSVPPLV EVMRGKSVIL DCTPTGTHDH YMLEWFLTDR SGARPRLASA EMQGSELQVT MHDTRGRSPP YQLDSQGRLV LAEAQVGDER DYVCVVRAGA AGTAEATARL NVFAKPEATE VSPNKGTLSV MEDSAQEIAT CNSRNGNPAP KITWYRNGQR LEVPVEMNPE GYMTSRTVRE ASGLLSLTST LYLRLRKDDR DASFHCAAHY SLPEGRHGRL DSPTFHLTLH YPTEHVQFWV GSPSTPAGWV REGDTVQLLC RGDGSPSPEY TLFRLQDEQE EVLNVNLEGN LTLEGVTRGQ SGTYGCRVED YDAADDVQLS KTLELRVAYL DPLELSEGKV LSLPLNSSAV VNCSVHGLPT PALRWTKDST PLGDGPMLSL SSITFDSNGT YVCEASLPTV PVLSRTQNFT LLVQGSPELK TAEIEPKADG SWREGDEVTL ICSARGHPDP KLSWSQLGGS PAEPIPGRQG WVSSSLTLKV TSALSRDGIS CEASNPHGNK RHVFHFGTVS PQTSQAVEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH
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Purification
- > 90% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM) (AA 32-548) (Active) protein (His tag)
BCAM Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM) (AA 26-541) (Active) protein (His tag)BCAM Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM) (Active) protein (His tag)BCAM Origin: Rat Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM) (AA 32-547) protein (His tag)BCAM Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))
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Alternative Name
- BCAM
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Background
- BCAM, also known as Basal cell adhesion molecule isoform 1, is an immunoglobulin superfamily protein that arises from alternate splicing of the Lutheran blood group molecule. This protein is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. Also, it interacts with integrin in sickle red cells, and may potentially play a role in vaso-occlusive episodes. Recombinant human BCAM, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 83.2 kDa (755aa)
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NCBI Accession
- NP_005572
Target
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