BCAM Protein (AA 32-548) (His tag)

Catalog No. ABIN7273931

Recombinant BCAM protein expressed in HEK-293 Cells.

Quick Overview for BCAM Protein (AA 32-548) (His tag) (ABIN7273931)

Target: BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 32-548

Purification tag / Conjugate: This BCAM protein is labelled with His tag.

Purpose: Cynomolgus BCAM Protein

Sequence: Glu32-Gly548

Characteristics: Recombinant Cynomolgus BCAM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu32-Gly548.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus BCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus BCAM is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

Alternative Name: BCAM

Background: Lutheran/basal cell adhesion molecule (Lu/BCAM) is a transmembrane adhesion molecule expressed by erythrocytes and endothelial cells that can interact with the extracellular matrix protein laminin-α5. In sickle cell disease, Lu/BCAM is thought to contribute to adhesion of sickle erythrocytes to the vascular wall, especially during vaso-occlusive crises.

Molecular Weight: 57.42 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

UniProt: A0A7N9CA99