C3 Protein (AA 672-748)

Catalog No. ABIN7314272

This Recombinant C3 protein is produced in Escherichia coli (E. coli).

Quick Overview for C3 Protein (AA 672-748) (ABIN7314272)

Target: C3 (Complement Component 3 (C3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 672-748

Purpose: Recombinant Human Complement C3 Protein is produced by E.coli expression system and the target gene encoding Ser672-Arg748 is expressed.

Sequence: SVQLTEKRMD KVGKYPKELR KCCEDGMREN PMRFSCQRRT RFISLGEACK KVFLDCCNYI TELRRQHARA SHLGLAR

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20  mM PB, 150  mM NaCl,  pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: C3 (Complement Component 3 (C3))

Alternative Name: C3

Gene ID: 718

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling