LC3B Protein (AA 1-125)

Catalog No. ABIN7314438

This Recombinant LC3B protein is produced in Escherichia coli (E. coli).

Quick Overview for LC3B Protein (AA 1-125) (ABIN7314438)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Mass Spectrometry (MS)

Purity: Greater than 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-125

Purpose: Recombinant Human LC3B Protein is produced by E.coli expression system and the target gene encoding Met1-Val125 is expressed.

Sequence: GSMPSEKTFK QRRTFEQRVE DVRLIREQHP TKIPVIIERY KGEKQLPVLD KTKFLVPDHV NMSELIKIIR RRLQLNANQA FFLLVNGHSM VSVSTPISEV YESEKDEDGF LYMVYASQET FGMKLSV

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20  mM Tris, 150  mM NaCl, 2  mM DTT,  pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: MAP1LC3B

Gene ID: 81631

UniProt: Q9GZQ8

Pathways: Autophagy