IgE Protein (His tag)
Quick Overview for IgE Protein (His tag) (ABIN6120361)
Target
See all IgE ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This IgE protein is labelled with His tag.
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Purpose
- Pig Immunoglobulin Heavy Constant Epsilon (IGHE) Protein
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Sequence
- SIQGPLVYPL TSCCKTGATS KTLGCLVRGY FPEPVTVTWD TGSLNSSTLT FPAVQDSSSS LYTVTSQVTI LGEWTNQKLT CSVAHAANTT IRTITGCTKN FTDPSLRFFY SSCDPHGDAQ ATIHLRCYIS GYTPGKMKVT WLVDGQEDRN LFSYTAPDQL EGKLASTYSE VNITQGQWAS QITYTCQVSY YGFIYEKHAL RCTAESEPRG VSAYLSPPTP LDLYVHKSPK LTCLVVDLAS SENVNLLWSR ENKGGVILPP PGPPVIKPQF NGTFSATSTL PVNVSDWIEG ETYYCNVTHP DLPKPILRSI SKGPGKRVTP EVYVLWSPDE LKKGRLTLTC LIQNFFPADI SVLWLRNDAP VQADRHSTTR PHKASDSLPS FFVYSRLVVS QSDWEQNKFA CEVIHEALPG SRTLQKEVSK NPGK
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Biological Activity Comment
- Not tested
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IgE (AA 209-428) protein (His-Avi Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 91-421) (Active) protein (His tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 198-421) (Active) protein (His-Avi Tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)Origin: Human Host: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, FACS
IgE (AA 1-429) protein (His tag)Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-432) protein (His tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-428) protein (His tag)Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % Sarcosyl, 5 % Trehalose.
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Preservative
- Dithiothreitol (DTT), Other preservative
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- IgE
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Alternative Name
- Immunoglobulin E
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Target Type
- Antibody
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Molecular Weight
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Calculated MW: 50.5 kDa
Observed MW (SDS-PAGE): 50 kDa -
UniProt
- K7ZJQ0
Target
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