Apo-B100 Protein (His tag)
This Recombinant Apo-B100 protein is expressed in Escherichia coli (E. coli).
Catalog No. ABIN6120377
$642.06
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Quick Overview for Apo-B100 Protein (His tag) (ABIN6120377)
Target
See all Apo-B100 Proteins
Apo-B100
(Apoprotein B100 (Apo-B100))
Protein Type
Recombinant
Origin
Human
Source
Escherichia coli (E. coli)
Application
Western Blotting (WB), SDS-PAGE (SDS)
Purity
> 90 %
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Purification tag / Conjugate
- This Apo-B100 protein is labelled with His tag.
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Purpose
- Human Apolipoprotein B-100 (APOB-100) Protein
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Sequence
- HLLSSS SSVIDALQYK LEGTTRLTRK RGLKLATALS LSNKFVEGSH NSTVSLTTKN MEVSVATTTK AQIPILRMNF KQELNGNTKS KPTVSSSMEF KYDFNSSMLY STAKGAVDHK LSLESLTSYF SIESSTKGDV KGSVLSREYS GTIASEANTY LNSKSTRSSV KLQGTSKIDD IWNLEVKE
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Biological Activity Comment
- Not tested
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 5 % Trehalose.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Apo-B100 (Apoprotein B100 (Apo-B100))
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Alternative Name
- Apolipoprotein B100
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Molecular Weight
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Calculated MW: 21.4 kDa
Observed MW (SDS-PAGE): 26 kDa Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.Gene ID
- 338
HGNC
- 603
OMIM
- 107730
UniProt
- P04114
Target
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