DSPP Protein (His tag)

Catalog No. ABIN6120517

This Recombinant DSPP protein is produced in Escherichia coli (E. coli).

Quick Overview for DSPP Protein (His tag) (ABIN6120517)

Target: DSPP (Dentin Sialophosphoprotein (DSPP))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This DSPP protein is labelled with His tag.

Purpose: Human Dentin Sialophosphoprotein (DSPP) Protein

Sequence: GSKWAEVGG KSFSTYSTLA NEEGNIEGWN GDTGKAETYG HDGIHGKEEN ITANGIQGQV SIIDNAGATN RSNTNGNTDK NTQNGDVGDA GHNEDVAVVQ EDGPQVAGSN NSTDNEDEIT ENSCRNEGNT SEITPQINSK RNGTKEAEVT PGTGEDAGLD NSDGSPSGNG ADEDEDEGSG DDEDEEAGNG KDSSNNSKGQ EGQDHGKEDD HDSSIGQNSD SKEYYDPEGK EDPHNEVDGD KTSKSE

Biological Activity Comment: Not tested

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.

Buffer: Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01 % Sarcosyl, 5 % Trehalose.

Storage: 4 °C,-80 °C

Storage Comment: Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: DSPP (Dentin Sialophosphoprotein (DSPP))

Alternative Name: Dentin Sialophosphoprotein

Molecular Weight: Calculated MW: 29.3 kDa
Observed MW (SDS-PAGE): 39 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.

Gene ID

1834

HGNC: 3054

OMIM: 125420

UniProt: Q9NZW4