Nodal Protein (His tag)
Quick Overview for Nodal Protein (His tag) (ABIN6125119)
Target
See all Nodal (NODAL) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Nodal protein is labelled with His tag.
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Purpose
- Human Nodal Homolog (NODAL) Protein
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Biological Activity Comment
- Not tested
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Nodal Homolog (NODAL) (Active) protein
NODAL Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Nodal Homolog (NODAL) (AA 275-346) protein (GST tag)NODAL Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % Sarcosyl, 5 % Trehalose and Proclin-300.
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Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Nodal (NODAL) (Nodal Homolog (NODAL))
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Alternative Name
- Nodal Homolog
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Molecular Weight
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Calculated MW: 40.4 kDa
Observed MW (SDS-PAGE): 40 kDa -
Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of Endopeptidase Activity
Target
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