FGF21 Protein (AA 30-210) (DYKDDDDK Tag)

Catalog No. ABIN7278716

Recombinant FGF21 protein expressed in HEK-293 Cells.

Quick Overview for FGF21 Protein (AA 30-210) (DYKDDDDK Tag) (ABIN7278716)

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 30-210

Purification tag / Conjugate: This FGF21 protein is labelled with DYKDDDDK Tag.

Purpose: FGF-21 (mouse) (rec.)

Specificity: The extracellular domain of mouse FGF-21 (aa 30-210) is fused at the N-terminus to a FLAG®-tag.

Characteristics: Protein. The extracellular domain of mouse FGF-21 (aa 30-210) is fused at the N-terminus to a FLAG®-tag. Source: HEK 293 cells. Endotoxin content: <0.06EU/μg purified protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in 1X PBS. Purity: >90 % (SDS-PAGE). Fibroblast growth factor 21 (FGF-21) is a member of the fibroblast growth factor family and has been identified as an important regulator of energy metabolism connecting nutrition, growth, reproduction and longevity. FGF-21 lacks the heparin-binding domain of most FGF proteins, allowing its secretion. FGF-21 is abundantly expressed in adipose tissues, liver and pancreas. In adipose tissue, FGF-21 promotes glucose uptake and oxidation and in liver it replenishes on fasting the tissues with fuel on low nutritional supply. It also induces lipolysis and ketogenesis. FGF-21 acts through FGF receptors (FGFR) associated with the auxiliary protein beta-Klotho. Recently, FGF-21 has been reported to interact directly with the brain circadian clock to coordinate activity and reproduction as part of the adaptation to fasting. Due to its multiple functions of normalizing glucose, lipid and energy homeostasis, FGF-21 represents an attractive novel therapy for type 2 diabetes mellitus and obesity.

Endotoxin Level: <0.06EU/μg purified protein (LAL test, Lonza).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in 1X PBS.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Alternative Name: FGF-21

Background:

Alternate Names/Synonyms: Fibroblast Growth Factor 21

Product Description: Fibroblast growth factor 21 (FGF-21) is a member of the fibroblast growth factor family and has been identified as an important regulator of energy metabolism connecting nutrition, growth, reproduction and longevity. FGF-21 lacks the heparin-binding domain of most FGF proteins, allowing its secretion. FGF-21 is abundantly expressed in adipose tissues, liver and pancreas. In adipose tissue, FGF-21 promotes glucose uptake and oxidation and in liver it replenishes on fasting the tissues with fuel on low nutritional supply. It also induces lipolysis and ketogenesis. FGF-21 acts through FGF receptors (FGFR) associated with the auxiliary protein beta-Klotho. Recently, FGF-21 has been reported to interact directly with the brain circadian clock to coordinate activity and reproduction as part of the adaptation to fasting. Due to its multiple functions of normalizing glucose, lipid and energy homeostasis, FGF-21 represents an attractive novel therapy for type 2 diabetes mellitus and obesity.

Molecular Weight: ~23kDa (SDS-PAGE)

NCBI Accession: NP_064397

Pathways: RTK Signaling