ICAM1 Protein (AA 28-480) (Fc Tag)

Catalog No. ABIN6253321

This Recombinant ICAM1 protein is expressed in CHO Cells.

Quick Overview for ICAM1 Protein (AA 28-480) (Fc Tag) (ABIN6253321)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Protein Type: Recombinant

Origin: Human

Source: CHO Cells

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 28-480

Purification tag / Conjugate: This ICAM1 protein is labelled with Fc Tag.

Purpose: CD54 [ICAM-1] (human):Fc (human) (rec.) (non-lytic)

Specificity: The extracellular domain of human CD54 [ICAM-1] (aa 28-480) is fused to the N-terminus of the Fc region of a mutant human IgG1.

Characteristics: Protein. The extracellular domain of human CD54 [ICAM-1] (aa 28-480) is fused to the N-terminus of the Fc region of a mutant human IgG1. Source: CHO cells. Endotoxin content: <0.06EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >98 % (SDS-PAGE). Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac1. CD54 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1beta and LPS. Soluble CD54 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

Endotoxin Level: <0.06EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Shows the biological function of the CD54 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54 [ICAM-1]

Target Type: Viral Protein

Background:

Alternate Names/Synonyms: ICAM1, Intercellular Adhesion Molecule 1

Product Description: Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac1. CD54 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1beta and LPS. Soluble CD54 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

NCBI Accession: NP_000192

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling