IL1A Protein (AA 113-271) (His tag)

Catalog No. ABIN7278696

This Recombinant IL1A protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL1A Protein (AA 113-271) (His tag) (ABIN7278696)

Target: IL1A (Interleukin 1 alpha (IL1A))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 113-271

Purification tag / Conjugate: This IL1A protein is labelled with His tag.

Purpose: IL-1alpha (human) (rec.) (His)

Specificity: Human IL-1alpha (aa 113-271) is fused at the C-terminus to a His-tag.

Characteristics: Protein. Human IL-1alpha (aa 113-271) is fused at the C-terminus to a His-tag. Source: E. coli. Endotoxin content: <0.1EU/μg protein (LAL test, Lonza). Lyophilized from a concentrated sterile solution containing 50  mM Tris-HCl buffer (  pH 8.0) and 100  mM NaCl. Purity: >98 % (SDS-PAGE). The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.

Endotoxin Level: <0.1EU/μg protein (LAL test, Lonza).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from a concentrated sterile solution containing 50  mM Tris-HCl buffer (  pH 8.0) and 100  mM NaCl.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1alpha

Background:

Alternate Names/Synonyms: Interleukin-1alpha, IL-1F1, IL-1A

Product Description: The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.

NCBI Accession: NP_000566

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints