IL1A Protein (AA 115-270) (His tag)

Catalog No. ABIN7320583

This Recombinant IL1A protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL1A Protein (AA 115-270) (His tag) (ABIN7320583)

Target: IL1A (Interleukin 1 alpha (IL1A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 115-270

Purification tag / Conjugate: This IL1A protein is labelled with His tag.

Purpose: Recombinant Mouse IL-1A/IL-1α Protein

Sequence: Ser115-Ser270

Characteristics: Recombinant Mouse Interleukin-1 alpha is produced by our E.coli expression system and the target gene encoding Ser115-Ser270 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce D10.G4.1 cells proliferation.The ED₅₀ for this effect is <5 pg/mL.The specific activity of recombinant mouse IL-1 alpha is > 2 x 10⁸ IU/mg.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1A/IL-1alpha

Background: Hematopoietin,IL1F,Hematopoietin-1,IL1,IL1-A,IL1F1,IL-1α,Preinterleukin 1 Alpha,Pro-Interleukin-1-Alpha,Il-1a,Interleukin-1 alpha,IL-1 alpha,Il1a,Mouse Interleukin-1 (IL-1) designates two proteins, IL-1α and IL-1β, which are the products of distinct genes, but recognize the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show approximately 25 % homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa.

Molecular Weight:

Calculated MW: 18.9 kDa

Observed MW: 20 kDa

UniProt: P01582

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints