PD-L1 Protein (AA 19-238)

Catalog No. ABIN6253363

This Recombinant PD-L1 protein is expressed in HEK-293 Cells.

Quick Overview for PD-L1 Protein (AA 19-238) (ABIN6253363)

Target: PD-L1 (CD274 (PD-L1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 19-238

Purpose: CD274 [B7-H1/PD-L1] (human) (rec.)

Specificity: The extracellular domain of human CD274 [B7-H1] (aa 19-238) is untagged.

Characteristics: Protein. The extracellular domain of human CD274 [B7-H1] (aa 19-238) is untagged. Source: HEK 293 cells. Endotoxin content: <0.01EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >95 % (SDS-PAGE). Programmed death ligand 1 (PD-L1, B7-H1 or CD274) is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. CD274 has been identified as one of two ligands for programmed death 1 (PD-1), a member of the CD28 family of immunoreceptors. CD274 is widely expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. CD274 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. CD274 expression is also induced in dendritic cells and keratinocytes after IFN-gamma stimulation. CD274 expression is also upregulated in a variety of tumor cell lines. Interaction of CD274 with PD-1 results in inhibition of TCR mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. The CD274 - PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

Endotoxin Level: <0.01EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274 [B7-H1/PD-L1]

Background:

Alternate Names/Synonyms: PD-L1, Programmed Cell Death 1 Ligand Protein 1, PDCD1 Ligand 1, B7-H1, PDL1

Product Description: Programmed death ligand 1 (PD-L1, B7-H1 or CD274) is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. CD274 has been identified as one of two ligands for programmed death 1 (PD-1), a member of the CD28 family of immunoreceptors. CD274 is widely expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. CD274 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. CD274 expression is also induced in dendritic cells and keratinocytes after IFN-gamma stimulation. CD274 expression is also upregulated in a variety of tumor cell lines. Interaction of CD274 with PD-1 results in inhibition of TCR mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. The CD274 - PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

Molecular Weight: ~35kDa (SDS-PAGE)

NCBI Accession: NP_054862

Pathways: Cancer Immune Checkpoints