CD27 Protein (AA 21-192) (Fc Tag)

Catalog No. ABIN6253439

Recombinant CD27 protein expressed in CHO Cells.

Quick Overview for CD27 Protein (AA 21-192) (Fc Tag) (ABIN6253439)

Target: CD27 (CD27 Molecule (CD27))

Protein Type: Recombinant

Origin: Human

Source: CHO Cells

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 21-192

Purification tag / Conjugate: This CD27 protein is labelled with Fc Tag.

Purpose: CD27 (human):Fc (human) (rec.)

Specificity: The extracellular domain of human CD27 (aa 21-192) is fused to the N-terminus of the Fc region of human IgG1.

Characteristics: Protein. The extracellular domain of human CD27 (aa 21-192) is fused to the N-terminus of the Fc region of human IgG1. Source: CHO cells. Endotoxin content: <0.06EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >98 % (SDS-PAGE). CD27 (TNFRSF7) is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B cell differentiation, activation and immunoglobulin synthesis. The CD27 receptor transduces signals and subsequently leads to the activation of NF-kappaB and MAPK8/JNK, mediated by the adaptor proteins TRAF2 and TRAF5. In addition, the proapoptotic protein SIVA is capable of binding the cytoplasmic tail of CD27 and exerts action in the process of apoptosis.

Endotoxin Level: <0.06EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Measured by its ability to inhibit the proliferation of mouse T cells induced by CD27 ligand and anti-CD3.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background:

Alternate Names/Synonyms: T Cell Activation Antigen CD27, T14, TNFRSF7, Tumor Necrosis Factor Receptor Superfamily Member 7, S152

Product Description: CD27 (TNFRSF7) is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B cell differentiation, activation and immunoglobulin synthesis. The CD27 receptor transduces signals and subsequently leads to the activation of NF-kappaB and MAPK8/JNK, mediated by the adaptor proteins TRAF2 and TRAF5. In addition, the proapoptotic protein SIVA is capable of binding the cytoplasmic tail of CD27 and exerts action in the process of apoptosis.

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints