COCH Protein (AA 25-132) (DYKDDDDK Tag)

Catalog No. ABIN6253494

This Recombinant COCH protein is produced in HEK-293 Cells.

Quick Overview for COCH Protein (AA 25-132) (DYKDDDDK Tag) (ABIN6253494)

Target: COCH (Cochlin (COCH))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 25-132

Purification tag / Conjugate: This COCH protein is labelled with DYKDDDDK Tag.

Purpose: Cochlin (human) (LCCL domain) (rec.)

Specificity: Human cochlin (LCCL domain) (aa 25-132) is fused at the N-terminus to a FLAG®-tag.

Cross-Reactivity: Human

Characteristics: Human cochlin (LCCL domain) (aa 25-132) is fused at the N-terminus to a FLAG®-tag.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: COCH (Cochlin (COCH))

Alternative Name: Cochlin

Background:

COCH-5B2 (LCCL domain)

Cochlin is an extracellular matrix protein expressed in the cochlea and vestibule of the inner ear. Mutations in the cochlin gene are linked to the autosomal-dominant DFNA9 syndrome, a disorder characterized by hearing loss. Cochlin is also expressed in follicular dendritic cells of the spleen and the lymph nodes. The N-terminus of cochlin contains a LCCL domain also present in Factor C, a serine protease of the horseshoe crab Limulus, involved in activating the coagulation cascade in response to LPS. A recent study showed that in response to inflammation, LPS or bacterial infection, cochlin is cleaved by the proteases aggrecanases and secreted into blood circulation. The secreted cochlin (LCCL domain) seems to have a role in local cytokine production, recruitment of immune effector cells and bacterial clearance. Cochlin is secreted as two forms p8 and p18, whereas p18 being the glycosylated form of p8.

Molecular Weight: ~8kDa and 18kDa

UniProt: O43405

Pathways: Sensory Perception of Sound