BID Protein (His tag)
Quick Overview for BID Protein (His tag) (ABIN6302423)
Target
See all BID ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This BID protein is labelled with His tag.
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Purpose
- Mouse BH3 Interacting Domain Death Agonist (Bid) Protein
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Sequence
- MDSEVSNGSG LGAEHITDLL VFGFLQSSGC TRQELEVLGR ELPVQAYWEA DLEDELQTDG SQASRSFNQG RIEPDSESQE EIIHNIARHL AQIGDEMDHN IQPTLVRQLA AQFMNGSLSE EDKRNCLAKA LDEVKTAFPR DMENDKAMLI MTMLLAKKVA SHAPSLLRDV FHTTVNFINQ NLFSYVRNLV RNEMD
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Biological Activity Comment
- Not tested
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BH3 Interacting Domain Death Agonist (BID) (AA 1-195) protein (His tag)
custom-made BID Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
BH3 Interacting Domain Death Agonist (BID) (Active) protein (His-GST)BID Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. ELISA Active
BH3 Interacting Domain Death Agonist (BID) (AA 1-195) protein (GST tag)BID Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- BID (BH3 Interacting Domain Death Agonist (BID))
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Alternative Name
- BH3 Interacting Domain Death Agonist
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Molecular Weight
- Calculated MW: 25.8 kDa
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UniProt
- P70444
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Pathways
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
Target
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