HYAL1 Protein (His tag)
Quick Overview for HYAL1 Protein (His tag) (ABIN2941979)
Target
See all HYAL1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This HYAL1 protein is labelled with His tag.
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Purpose
- Human Hyaluronoglucosaminidase 1 (HYAL1) Protein
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Sequence
- FRGPLLPNR PFTTVWNANT QWCLERHGVD VDVSVFDVVA NPGQTFRGPD MTIFYSSQLG TYPYYTPTGE PVFGGLPQNA SLIAHLARTF QDILAAIPAP DFSGLAVIDW EAWRPRWAFN WDTKDIYRQR SRALVQAQHP DWPAPQVEAV AQDQFQGAAR AWMAGTLQLG RALRPRGLWG FYGFPDCYNY DFLSPNYTGQ CPSGIRAQND QLGWLWGQSR ALYPSIYMPA VLEGTGKSQM YVQHRVAEAF RVAVAAGDPN LPVLPYVQIF YDTTNHFLPL DELEHSLGES AAQGAAGVVL WVSWENTRTK ESCQAIKEYM DTTLGPFILN VTSGALLCSQ ALCSGHGRCV RRTSHPKALL LLNPASFSIQ LTPGGGPLSL RGALSLEDQA QMAVEFKCRC YPGWQAPWCE RKSMW
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Biological Activity Comment
- Not tested
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Hyaluronidase-1 (HYAL1) (AA 1-253) protein (GST tag)
HYAL1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Hyaluronidase-1 (HYAL1) (AA 22-435) protein (His tag)HYAL1 Origin: Human Host: Yeast Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
Hyaluronidase-1 (HYAL1) protein (His tag)HYAL1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB
Hyaluronidase-1 (HYAL1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)HYAL1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 1 mM DTT, 5 % Trehalose and Proclin-300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- HYAL1 (Hyaluronidase-1 (HYAL1))
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Alternative Name
- Hyaluronoglucosaminidase 1
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Molecular Weight
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Calculated MW: 47.7 kDa
Observed MW: 43 kDa -
UniProt
- Q12794
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Pathways
- Glycosaminoglycan Metabolic Process
Target
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