JTB Protein (AA 31-105) (His tag)

Catalog No. ABIN6387469

This Recombinant JTB protein is expressed in Escherichia coli (E. coli).

Quick Overview for JTB Protein (AA 31-105) (His tag) (ABIN6387469)

Target: JTB (Jumping Translocation Breakpoint (JTB))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 85 %

Product Details

Protein Characteristics: AA 31-105

Purification tag / Conjugate: This JTB protein is labelled with His tag.

Sequence: EAPVQEE KLSASTSNLP CWLVEEFVVA EECSPCSNFR AKTTPECGPT GYVEKITCSS SKRNEFKSCR SALMEQRL

Purification: > 85% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: JTB (Jumping Translocation Breakpoint (JTB))

Alternative Name: JTB

Background: Jumping translocation breakpoint, also known as JTB, is required for normal cytokinesis during mitosis. This protein plays a role in the regulation of cell proliferation. This protein may be a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. It lnhibits apoptosis induced by TGFB1. Overexpression induces swelling of mitochondria and reduces mitochondrial membrane potential. Recombinant human JTB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 10.7 kDa (98aa) confirmed by MALDI-TOF

NCBI Accession: NP_006685