MAP4K3 Protein (AA 1-321) (His tag)
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- Target See all MAP4K3 Proteins
- MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-321
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Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MAP4K3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
- Sequence
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MTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL - Purity
- > 95% by SDS-PAGE
- Biological Activity Comment
- Specific activity is > 70unit/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.
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Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) (AA 1-894) protein (His tag)
custom-made MAP4K3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) (AA 1-894) protein (His tag)custom-made MAP4K3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) (AA 1-321) protein (His tag)MAP4K3 Origin: E. coli Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) protein (Myc-DYKDDDDK Tag)MAP4K3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))
- Alternative Name
- glk (MAP4K3 Products)
- Background
- Glk belongs to the bacterial glucokinase family. This protein is not highly important in E. coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E. coli glk protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 37.1 kDa (344aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_416889
- UniProt
- P0A6V8
- Pathways
- MAPK Signaling
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