NME2 Protein (AA 1-152)
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- Target See all NME2 Proteins
- NME2 (Nucleoside diphosphate kinase B (NME2))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-152
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE
- Purity
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 1,800 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
- Top Product
- Discover our top product NME2 Protein
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Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (GST tag)
NME2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His tag)NME2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (His tag)NME2 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nucleoside diphosphate kinase B (NME2) (AA 51-152) protein (GST tag)NME2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nucleoside diphosphate kinase B (NME2) (AA 1-152) protein (Strep Tag)Crystallography grade NME2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Nucleoside diphosphate kinase B (NME2) proteinNME2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % ELISA, SDS, WB, IP
Nucleoside diphosphate kinase B (NME2) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)NME2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- NME2 (Nucleoside diphosphate kinase B (NME2))
- Alternative Name
- NME2 (NME2 Products)
- Synonyms
- NDKB Protein, NDPK-B Protein, NDPKB Protein, NM23-H2 Protein, NM23B Protein, PUF Protein, nm23-2 Protein, p18-12d Protein, nm23-M2 Protein, NM23-C2 Protein, NME/NM23 nucleoside diphosphate kinase 2 Protein, non-metastatic cells 2, protein (NM23B) expressed in Protein, NME2 Protein, Nme2 Protein
- Background
- NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 17.2 kDa (152aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_001018149
- UniProt
- P22392
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