AKR1C1 Protein (AA 1-323)
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- Target See all AKR1C1 (DDH) Proteins
- AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-323
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.
- Top Product
- Discover our top product DDH Protein
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Dihydrodiol Dehydrogenase (DDH) (AA 1-323) protein (His tag)
DDH Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Dihydrodiol Dehydrogenase (DDH) (AA 1-323) protein (GST tag)DDH Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Dihydrodiol Dehydrogenase (DDH) (AA 1-110) protein (GST tag)DDH Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Dihydrodiol Dehydrogenase (DDH) (AA 1-323) (Active) protein (His tag)DDH Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Dihydrodiol Dehydrogenase (DDH) (AA 1-323) protein (Strep Tag)Crystallography grade DDH Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.5) containing 0.1M NaCl, 20 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- AKR1C1 (DDH) (Dihydrodiol Dehydrogenase (DDH))
- Alternative Name
- Aldo-keto reductase family 1 member C1 (DDH Products)
- Synonyms
- 2-ALPHA-HSD Protein, 20-ALPHA-HSD Protein, C9 Protein, DD1 Protein, DD1/DD2 Protein, DDH Protein, DDH1 Protein, H-37 Protein, HAKRC Protein, HBAB Protein, MBAB Protein, aldo-keto reductase family 1 member C1 Protein, aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) Protein, AKR1C1 Protein
- Background
- AKR1C1 also known as Aldo-keto reductase family 1 member C1, is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant human AKR1C1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 36.7kDa (323aa)
- NCBI Accession
- NP_001344
- UniProt
- Q04828
- Pathways
- Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process
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