CNTF Protein (AA 1-200)
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- Target See all CNTF Proteins
- CNTF (Ciliary Neurotrophic Factor (CNTF))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-200
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is less than or equal to 500 ng/ml.
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Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)
custom-made CNTF Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ciliary Neurotrophic Factor (CNTF) (AA 1-198) protein (His tag)custom-made CNTF Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ciliary Neurotrophic Factor (CNTF) proteinCNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)CNTF Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)CNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (GST tag)CNTF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CNTF (Ciliary Neurotrophic Factor (CNTF))
- Alternative Name
- CNTF (CNTF Products)
- Background
- CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF protein was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 22.9 kDa (200aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_000605
- UniProt
- P26441
- Pathways
- JAK-STAT Signaling
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