CNTF Protein (AA 1-200)
Quick Overview for CNTF Protein (AA 1-200) (ABIN6387771)
Target
See all CNTF ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-200
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Sequence
- MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
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Purification
- > 90% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤500ng/ml.
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Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)
custom-made CNTF Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ciliary Neurotrophic Factor (CNTF) (AA 1-198) protein (His tag)custom-made CNTF Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)CNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His-IF2DI Tag)CNTF Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 2-198) protein (His-IF2DI Tag)CNTF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (GST tag)CNTF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) (Active) protein (His tag)CNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, AcA Active
Ciliary Neurotrophic Factor (CNTF) (AA 4-196), (partial) protein (His tag)CNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- CNTF (Ciliary Neurotrophic Factor (CNTF))
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Alternative Name
- CNTF
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Background
- CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF protein was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 22.9 kDa (200aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_000605
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Pathways
- JAK-STAT Signaling
Target
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