PTH Protein (AA 32-115)
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- Target See all PTH Proteins
- PTH (Parathyroid Hormone (PTH))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 32-115
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 for this effect is less than or equal to 50 ng/ml.
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- Discover our top product PTH Protein
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Parathyroid Hormone (PTH) (Active) protein
PTH Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Parathyroid Hormone (PTH) (AA 32-115) protein (Fc Tag)PTH Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Parathyroid Hormone (PTH) (AA 32-115) (Active) protein (MBP tag,His tag)PTH Origin: Rat Host: Escherichia coli (E. coli) > 90 % CC, AcA Active
Parathyroid Hormone (PTH) (AA 32-115) protein (His-IF2DI Tag)PTH Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Parathyroid Hormone (PTH) (AA 32-115) protein (His tag)PTH Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Parathyroid Hormone (PTH) (AA 1-115) protein (GST tag)PTH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Parathyroid Hormone (PTH) (AA 32-115) protein (GST tag)PTH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In phosphate-buffered Saline( pH 7.4) containing 2 mM EDTA
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PTH (Parathyroid Hormone (PTH))
- Alternative Name
- PTH (PTH Products)
- Synonyms
- PTH1 Protein, Pthp Protein, PTH-(1-84) Protein, Pth1 Protein, Pthr1 Protein, PTH Protein, parathyroid hormone Protein, parathyroid hormone S homeolog Protein, PTH Protein, Pth Protein, pth.S Protein
- Target Type
- Hormone
- Background
- Human parathyroid hormone(hPTH) is an 84 amino acid residue peptide and one of the main regulators in maintenance of calcium homeostasis. It acts primarily on kidney and bone cells stimulating calcium back resorption or calcium mobilization, respectively. The whole mechanism is still under discussion, but low-dose hPTH triggers cyclic AMP-dependent protein kinase in some populations of bone cells bearing PTH receptors, which stimulates the proliferation of osteoblasts. Human parathyroid hormone(amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques
- Molecular Weight
- 9.55 kDa (85 aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_000306
- UniProt
- P01270
- Pathways
- cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process
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