POGLUT1 Protein (AA 24-392) (His tag)

Catalog No. ABIN6387809

Recombinant POGLUT1 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for POGLUT1 Protein (AA 24-392) (His tag) (ABIN6387809)

Target: POGLUT1 (Protein O-Glucosyltransferase 1 (POGLUT1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 24-392

Purification tag / Conjugate: This POGLUT1 protein is labelled with His tag.

Sequence: RQKESGSKWK VFIDQINRSL ENYEPCSSQN CSCYHGVIEE DLTPFRGGIS RKMMAEVVRR KLGTHYQITK NRLYRENDCM FPSRCSGVEH FILEVIGRLP DMEMVINVRD YPQVPKWMEP AIPVFSFSKT SEYHDIMYPA WTFWEGGPAV WPIYPTGLGR WDLFREDLVR SAAQWPWKKK NSTAYFRGSR TSPERDPLIL LSRKNPKLVD AEYTKNQAWK SMKDTLGKPA AKDVHLVDHC KYKYLFNFRG VAASFRFKHL FLCGSLVFHV GDEWLEFFYP QLKPWVHYIP VKTDLSNVQE LLQFVKANDD VAQEIAERGS QFIRNHLQMD DITCYWENLL SEYSKFLSYN VTRRKGYDQI IPKMLKTELL EHHHHHH

Purification: > 90% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: POGLUT1 (Protein O-Glucosyltransferase 1 (POGLUT1))

Alternative Name: Protein O-glucosyltransferase 1/POGLUT1

Background: POGLUT1, also known as protein O-glucosyltransferase 1, is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER) -retaining glucosyltransferase that adds glucose to serine residues within the consensus sequence of C1-X-S-X-P-C2 in Notch EGF repeats, thereby regulating cell-fate decisions. Recombinant human POGLUT1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 44.5 kDa (377aa)

NCBI Accession: NP_689518

Pathways: Notch Signaling