ALPL Protein (AA 19-503) (His tag)
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- Target See all ALPL Proteins
- ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 19-503
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Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This ALPL protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVT AARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTR CNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQL MHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GLDLISIWKS FKPRHKHSHY VWNRTELLAL DPSRVDYLLG LFEPGDMQYE LNRNNLTDPS LSEMVEVALR ILTKNLKGFF LLVEGGRIDH GHHEGKAKQA LHEAVEMDQA IGKAGAMTSQ KDTLTVVTAD HSHVFTFGGY TPRGNSIFGL APMVSDTDKK PFTAILYGNG PGYKVVDGER ENVSMVDYAH NNYQAQSAVP LRHETHGGED VAVFAKGPMA HLLHGVHEQN YIPHVMAYAS CIGANLDHCA WAGSGLEHHH HHH
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 46,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.
- Top Product
- Discover our top product ALPL Protein
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Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 19-113) protein (His-IF2DI Tag)
ALPL Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 1-524) protein (GST tag)ALPL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 91-200) protein (GST tag)ALPL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))
- Alternative Name
- Alpl (ALPL Products)
- Synonyms
- ALP Protein, iap Protein, alpl Protein, AP-TNAP Protein, APTNAP Protein, HOPS Protein, TNAP Protein, TNSALP Protein, Akp-2 Protein, Akp2 Protein, PHOA Protein, TNS-AP Protein, alkaline phosphatase, liver/bone/kidney L homeolog Protein, alkaline phosphatase, liver/bone/kidney Protein, alkaline phosphatase, intestinal, gene 2 Protein, alkaline phosphatase, liver/bone/kidney pseudogene Protein, alpl.L Protein, ALPL Protein, alpi.2 Protein, LOC100031702 Protein, Alpl Protein
- Background
- Alpl, also known as alkaline phosphatase, tissue-nonspecific isozyme, is one of alkaline phosphatases. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue-nonspecific). The product of this gene is a membrane-bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. A proposed function of this form of the enzyme is matrix mineralization. Recombinant mouse Alpl, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 54.5kDa (493aa) 50-70KDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_031457
- UniProt
- P09242
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