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ACP5 Protein (AA 22-325)

This Recombinant ACP5 protein is produced in Baculovirus infected Insect Cells.
Catalog No. ABIN6387961

Quick Overview for ACP5 Protein (AA 22-325) (ABIN6387961)

Target

See all ACP5 Proteins
ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 8
  • 4
  • 3
  • 3
  • 1
Human

Source

  • 8
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
Baculovirus infected Insect Cells

Application

SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity

> 90% by SDS-PAGE
  • Protein Characteristics

    AA 22-325

    Sequence

    ATPALRFVAV GDWGGVPNAP FHTAREMANA KEIARTVQIL GADFILSLGD NFYFTGVQDI NDKRFQETFE DVFSDRSLRK VPWYVLAGNH DHLGNVSAQI AYSKISKRWN FPSPFYRLHF KIPQTNVSVA IFMLDTVTLC GNSDDFLSQQ PERPRDVKLA RTQLSWLKKQ LAAAREDYVL VAGHYPVWSI AEHGPTHCLV KQLRPLLATY GVTAYLCGHD HNLQYLQDEN GVGYVLSGAG NFMDPSKRHQ RKVPNGYLRF HYGTEDSLGG FAYVEISSKE MTVTYIEASG KSLFKTRLPR RARP

    Endotoxin Level

    < 1 EU per 1ug of protein (determined by LAL method)

    Biological Activity Comment

    Specific activity is >10,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C
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  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    Bioactivity Validated

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
  • Target

    ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

    Alternative Name

    TRACP/PAP/ACP5

    Background

    ACP5, also known as tartrate-resistant acid phosphatase type 5, is involved in osteopontin and bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Recombinant human ACP5 was expressed in insect cell and purified by using conventional chromatography techniques.

    Molecular Weight

    34.3 kDa (304aa)

    NCBI Accession

    NP_001104505

    UniProt

    P13686

    Pathways

    Transition Metal Ion Homeostasis
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