ACP5 Protein (His tag)

Catalog No. ABIN7194093

Product Details

Target: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This ACP5 protein is labelled with His tag.

Purpose: Recombinant Mouse ACP5/TRAP Protein (His Tag)(Active)

Sequence: Met 1-Pro 327

Characteristics: A DNA sequence encoding the pro form of mouse ACP5 (NP_031414.1) (Met 1-Pro 327) was fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is >9,000 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Alternative Name: ACP5/TRAP (ACP5 Products)

Synonyms: SPENCDI Protein, TRAP Protein, acp5 Protein, sb:cb576 Protein, zgc:63825 Protein, wu:fb19f01 Protein, wu:fb30b03 Protein, wu:fi14e01 Protein, wu:fj66f03 Protein, MGC78938 Protein, MGC89674 Protein, TR-AP Protein, zgc:92339 Protein, TRACP Protein, TTRRAP Protein, Trap Protein, UF Protein, acid phosphatase 5, tartrate resistant Protein, acid phosphatase 5a, tartrate resistant Protein, acid phosphatase 5, tartrate resistant S homeolog Protein, tartrate resistant acid phosphatase Protein, Tartrate-resistant acid phosphatase type 5 Protein, tartrate-resistant acid phosphatase type 5 Protein, acid phosphatase 5b, tartrate resistant Protein, ACP5 Protein, acp5a Protein, acp5.S Protein, acp5 Protein, NAEGRDRAFT_81291 Protein, ppa5 Protein, LOC100282899 Protein, acp5b Protein, Acp5 Protein

Background:

Background: Tartrate-resistant acid phosphatase (TRACP) or acid phosphatase 5, tartrate resistant (ACP5 or TRAP) is a glycosylated monomeric metalloenzyme expressed in mammals. TRACP is associated with osteoblast migration to bone resorption sites, and, once there, TRACP is believed to initiate osteoblast differentiation, activation, and proliferation. TRACP once considered to be just a histochemical marker of osteoclasts is now recognised to be a molecule of widespread occurrence with functions in both the skeleton and the immune system. Two forms of TRACP circulate in human blood, TRACP 5a derived from macrophages and dendritic cells, and TRACP-5b derived from osteoclasts. Recent data have demonstrated the utility of TRACP-5b as a marker of osteoclast number and bone resorption, and serum TRACP-5a as a marker of inflammatory conditions. TRACP is expressed by osteoclasts, macrophages, dendritic cells and a number of other cell types. It has a critical role in many biological processes including skeletal development, collagen synthesis and degradation, the mineralisation of bone, cytokine production by macrophages and dendritic cells, macrophage recruitment, dendritic cell maturation and a role in the development of Th1 responses.

Synonym: TRACP,TRAP

Molecular Weight: 35.8 kDa

NCBI Accession: NP_031414

Pathways: Transition Metal Ion Homeostasis