EGF Protein (AA 977-1029) (His tag)
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- Target See all EGF Proteins
- EGF (Epidermal Growth Factor (EGF))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 977-1029
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This EGF protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Activity Assay (AcA)
- Sequence
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NSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR - Purity
- > 95% by SDS-PAGE
- Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Measured in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 range ≤ 1ng/ml.
- Top Product
- Discover our top product EGF Protein
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Epidermal Growth Factor (EGF) (AA 971-1023) protein
EGF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (Active) proteinEGF Origin: Human Host: Escherichia coli (E. coli) > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
Epidermal Growth Factor (EGF) (AA 971-1023) protein (Fc Tag)EGF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)EGF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)EGF Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (Active) proteinEGF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Epidermal Growth Factor (EGF) (AA 974-1026) protein (His tag)EGF Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)EGF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Epidermal Growth Factor (EGF) proteinEGF Origin: Rat Host: Escherichia coli (E. coli) Recombinant 95,00% SDS
Epidermal Growth Factor (EGF) proteinEGF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl( pH 8.0) containing 0.1M NaCl, 2 mM DTT, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- EGF (Epidermal Growth Factor (EGF))
- Alternative Name
- EGF (EGF Products)
- Background
- Egf, also known as Pro-epidermal growth factor isoform1, is a 8. 6 kDa globular protein containing 77 amino acids residues, including 3 intra-molecular disulfide bonds. Egf is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, Egf has been shown to inhibit gastric secretion, and to be involved in wound healing. Egf signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant mouse Egf protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional column chromatography
- Molecular Weight
- 8.6 kDa (77aa) Confirmed by MALDI-TOF
- NCBI Accession
- NP_034243
- Pathways
- NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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