GST Protein (AA 1-218)

Catalog No. ABIN6388215

Product Details

Target: GST (Glutathione S Transferase (GST))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-218

Origin: Schistosoma japonicum

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPK

Purity: > 90 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 30 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In Phosphate Buffer Saline containing 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: GST (Glutathione S Transferase (GST))

Alternative Name: GST (GST Products)

Synonyms: GST Protein, GST 13-13 Protein, GST13 Protein, GST13-13 Protein, GSTK1-1 Protein, hGSTK1 Protein, 1500002K10Rik Protein, Gst Protein, Ptgds2 Protein, glutathione S-transferase kappa 1 Protein, microsomal glutathione S-transferase 1 Protein, hematopoietic prostaglandin D synthase Protein, GSTK1 Protein, Mgst1 Protein, Hpgds Protein

Background: GST, also known as, Glutathione S-transferase, represents a major group of detoxification enzymes. GST acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. GST was originally separated from Schistosomajaponicum but currently isolated from recombinant E.coli source. Recombinant Schistosoma japonicum GST was expressed in E.coli and purified by conventional chromatography techniques.

Molecular Weight: 25.4 kDa (218aa)

UniProt: P08515