SHP1 Protein (AA 243-541)
Quick Overview for SHP1 Protein (AA 243-541) (ABIN666674)
Target
See all SHP1 (PTPN6) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 243-541
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Sequence
- MGFWEEFESL QKQEVKNLHQ RLEGQRPENK GKNRYKNILP FDHSRVILQG RDSNIPGSDY INANYIKNQL LGPDENAKTY IASQGCLEAT VNDFWQMAWQ ENSRVIVMTT REVEKGRNKC VPYWPEVGMQ RAYGPYSVTN CGEHDTTEYK LRTLQVSPLD NGDLIREIWH YQYLSWPDHG VPSEPGGVLS FLDQINQRQE SLPHAGPIIV HCSAGIGRTG TIIVIDMLME NISTKGLDCD IDIQKTIQMV RAQRSGMVQT EAQYKFIYVA IAQFIETTKK KLEVLQSQKG QESEYGNITY
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Characteristics
- SHP-1, Human, Recombinant, E.coli
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Purification
- > 95% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 8,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C
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Protein-tyrosine Phosphatase 1C (PTPN6) (AA 1-595) protein (His tag)
custom-made PTPN6 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein-tyrosine Phosphatase 1C (PTPN6) (AA 1-595) protein (His tag)custom-made PTPN6 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein-tyrosine Phosphatase 1C (PTPN6) (AA 5-163) protein (His-IF2DI Tag)PTPN6 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Protein-tyrosine Phosphatase 1C (PTPN6) (AA 1-597) protein (GST tag)PTPN6 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA, PAA
Protein-tyrosine Phosphatase 1C (PTPN6) (AA 207-597) (Active) protein (GST tag,His tag)PTPN6 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE Active
Protein-tyrosine Phosphatase 1C (PTPN6) (Active) proteinPTPN6 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 25 mM Tris-HCl buffer ( pH 7.5) containing 2 mM beta-mercaptoethanol, 1 mM EDTA 1 mM DTT, 20 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))
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Alternative Name
- SHP-1
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Background
- The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid (Met) is attached at N-terminus.
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Molecular Weight
- 34.3 kDa (300aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_002822
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Pathways
- JAK-STAT Signaling, TCR Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling
Target
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