Midkine Protein (AA 21-143) (His tag)
Quick Overview for Midkine Protein (AA 21-143) (His tag) (ABIN666781)
Target
See all Midkine (MDK) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 21-143
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Purification tag / Conjugate
- This Midkine protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MVAKKKDKVK KGGPGSECAE WAWGPCTPSS KDCGVGFREG TCGAQTQRIR CRVPCNWKKE FGADCKYKFE NWGACDGGTG TKVRQGTLKK ARYNAQCQET IRVTKPCTPK TKAKAKAKKG KGKD
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Characteristics
- Midkine, 21-143 aa, Human, His-tagged, Recombinant, E.coli
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Purification
- > 90% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Midkine (Neurite Growth-Promoting Factor 2) (MDK) (Active) protein
MDK Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 1-143) protein (GST tag)MDK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-140) protein (His-IF2DI Tag)MDK Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 23-143) protein (Fc Tag)MDK Origin: Cynomolgus Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-143) protein (Fc Tag)MDK Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 23-140) (Active) protein (His tag)MDK Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE Active
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-143) (Active) protein (His-Avi Tag)MDK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 2 mM DTT, and 20 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))
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Alternative Name
- Midkine
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Background
- Midkine, also known as Neurite Growth-promoting Factor 2, is a basic heparin-binding growth factor of low molecular weight, a member of the NEGF family whose founding member is pleiotrophin. This protein exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Midkine is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. Recombinant human Midkine protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molecular Weight
- 15.7 kDa (144aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_002382
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Pathways
- RTK Signaling, M Phase, Skeletal Muscle Fiber Development
Target
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