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DnaK (AA 508-638) Protein

This Recombinant protein is expressed in Escherichia coli (E. coli).
Catalog No. ABIN666807

Quick Overview for DnaK (AA 508-638) Protein (ABIN666807)

Target

DnaK

Protein Type

Recombinant

Origin

  • 4
  • 2
  • 1
E. coli

Source

  • 7
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS)

Purity

> 95 % by SDS - PAGE
  • Protein Characteristics

    AA 508-638

    Characteristics

    Dnak(508-638), E.coli, Recombinant, E.coli
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  • Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/ml (determined by Bradford assay)

    Buffer

    Liquid. 25 mM Tris-HCl, pH7.5, 100mM NaCl, 1 mM DTT, 10%Glycerol.

    Storage

    4 °C
  • Target

    DnaK

    Alternative Name

    Dnak (508-638)

    Background

    DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak(residues 508-638) of the substrate binding domain is alpha-helical and appears to act as a lid covering the substrate binding cleft. DnaK(amino acid 508-638) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus. Synonyms: Dnak (508-638), A lid covering the substrate, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, Chaperone protein dnaK, Chaperone Hsp 70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein,. NCBI no.: NP_414555

    Molecular Weight

    14.6kDa (132aa)
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